TFIIA-α (E311) Polyclonal Antibody-BS2282

TFIIA-α (E311) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2282-50, BS2282-100

Sizes: 50µl, 100µl

Swiss-Prot: P52655

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP, one of the first steps in transcription initiation, may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-α, TFIIA-β and TFIIA-γ, and it interacts with both TBP and a TAF (TBP-associated factor). It has been demonstrated that the basic region of TBP is essential for TFIIA-dependent function of TBP.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TFIIA-α (E311) polyclonal antibody detects endogenous levels of TFIIA-α protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TFIIA-α (E311) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: SP2/0 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of TFIIA-α (E311) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 42 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Transcription initiation factor IIA subunit 1; General transcription factor IIA subunit 1; TFIIAL; Transcription initiation factor TFIIA 42 kDa subunit; TFIIA-42; Transcription initiation factor IIA alpha chain; TFIIA p35 subunit; Transcription initiation factor IIA beta chain; TFIIA p19 subunit; GTF2A1; TF2A1

Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human TFIIA-α.

Conjugate: Unconjugated

Modification: Unmodified