Product Description

TFIIA-α (E311) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS2282-50, BS2282-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a mμltiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP, one of the first steps in transcription initiation, may be regμlated by TFIIA. TFIIA consists of three subunits designated TFIIA-α, TFIIA-β and TFIIA-γ, and it interacts with both TBP and a TAF (TBP-associated factor). It has been demonstrated that the basic region of TBP is essential for TFIIA-dependent function of TBP.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Transcription initiation factor IIA subunit 1, General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42, Transcription initiation factor IIA alpha chain, TFIIA p35 subunit, Transcription initiation factor IIA beta chain, TFIIA p19 subunit, GTF2A1, TF2A1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human TFIIA-α.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TFIIA-α (E311) polyclonal antibody detects endogenous levels of TFIIA-α protein.

Molecular Weight: ~ 50-60 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P52655

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only