Product Description

TFIIB Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0045-50, MB0045-100

Product: Mouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q00403

Host: Mouse

Reactivity: Human

Applications: WB, IHC ICC

All Applications: WB: 1:1000
ICC: 1:50~200
IHC: 1:500

Background: Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the “TATA” element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of TFIIB and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size: 35KDa
Observed band size: 37KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot detection of TFIIB in Jurkat, MCF7 and Hela cell lysates using TFIIB antibody (1:1000 diluted)., IHC of paraffin-embedded human colon using anti- TFIIB diluted 1/500-1/1000., Immunocytochemistry of HeLa cells using anti- TFIIB antibody diluted 1:50.

Alternative Name: TF2B; TFIIB; S300-II; Transcription initiation factor IIB

Immunogen: Recombinant full length Human TFIIB.

Conjugate: Unconjugated

Modification: Unmodified