Product Description

TFIIB Monoclonal Antibody

Catalogue Numbers: MB66646-50, MB66646-100

Sizes: 50µl, 100µl

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q00403

Host: Mouse

Reactivity: Human, Mouse, Monkey

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: TFIIB (also known as GTF2B, TF2B and general transcription factor IIB) is a ubiquitously expressed transcription factor that plays a central role in the assembly of the transcription pre-initiation complex through direct recruitment of RNA polymerase II. TFIIB functions as a bridge between promoter-bound TFIID and RNA polymerase II. In addition to interacting with promoter-bound TFIID and TFIIA, TFIIB makes extensive contacts with the core promoter via two independent DNA-binding modules. TFIIB may also be a target of transcriptional activator proteins that act to stimulate pre-initiation complex assembly.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of TFIIB protein.

Extra Notes: Western blot analysis of TFIIB expression in Jurkat (A), MCF7 (B), COS7 (C), NIH3T3 (D), Hela (E) whole cell lysates.

Bioworld Molecular Weight: ~ 35 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: TF2B; TFIIB; Transcription initiation factor IIB; General transcription factor TFIIB; S300-II

Immunogen: Purified recombinant human TFIIB protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified