TFIIE-β (K183) Polyclonal Antibody-BS1974

TFIIE-β (K183) Polyclonal Antibody

Catalogue Numbers: BS1974-50, BS1974-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29084

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The general transcription factor TFIIE consists of two subunits, α and β, and plays essential roles in transcription.Structure-function studies indicate that TFIIE has three-winged helix (WH) motifs, with one in TFIIEα and two in TFIIEβ. by binding to the clamp region of RNA polymerase II, TFIIEα-WH promotes the conformational change that transforms the promoter-bound inactive preinitiation complex to the active complex.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: TFIIE-β (K183) pAb detects endogenous levels of TFIIE-β protein.

Extra Notes: Western blot (WB) analysis of TFIIE-β (K183) pAb at 1:500 dilution
Lane1:SGC7901 whole cell lysate(40ug)
Lane2:HCT116 whole cell lysate(40ug)
Lane3:The Testis tissue lysate of Mouse(40ug)

Bioworld Molecular Weight: ~ 33 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Transcription initiation factor IIE subunit beta; TFIIE-beta; General transcription factor IIE subunit 2; GTF2E2; TF2E2

Immunogen: Synthetic peptide, corresponding to amino acids 153-213 of Human TFIIE-β.

Conjugate: Unconjugated

Modification: Unmodified