TFIIE-β Polyclonal Antibody-BS5964

TFIIE-β Polyclonal Antibody

Catalogue Numbers: BS5964-50, BS5964-100

Sizes: 50µl, 100µl

Alternative Name: Transcription initiation factor IIE subunit beta; TFIIE-beta; General transcription factor IIE subunit 2; GTF2E2; TF2E2; TFIIE β; TFIIEβ;

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29084

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequencespecific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcriptional start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Human TFIIE consists of two subunits of 56 kDa and 34 kDa molecular weight, respectively. The structure of TFIIE appears to be a heterotetramer(α2 β2) both subunits being required for optimal basal-level transcription.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: TFIIE-β polyclonal antibody detects endogenous levels of TFIIE-β protein.

Bioworld Molecular Weight: ~ 33 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TFIIE-β polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 193-238 of Human TFIIE-β.

Conjugate: Unconjugated

Modification: Unmodified