Product Description

TG1019 (R251) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3237-50, BS3237-100

Sizes: 50µl, 100µl

Swiss-Prot: Q8TDS5

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: TG1019 is a G protein-coupled receptor that functions as a chemoattractant for eosinophils and neutrophils. TG1019 is expressed in various tissues except the brain, with the highest expression in liver, kidney, peripheral leukocyte, lung and spleen. TG1019 interacts with the protein OXE to form TG1019/OXE, a Gα i/o protein-coupled receptor. Signals from TG1019 are transduced via a Gα i/o protein to PLC/calcium, MEK/ERK and PI3K/Akt pathways. Signal transduction from TG1019 following stimulation with 5-oxo-6E,8Z,11Z,14Z eicosatetraenoic acid (5-oxo-ETE) induces intracellular calcium mobilization and rapid activation of MEK/ERK and PI3K/Akt pathways. TG1019 also may play a role in downregulating cAMP production.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TG1019 (R251) polyclonal antibody detects endogenous levels of TG1019 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TG1019 (R251) pAb at 1:500 dilution
Lane 1: 3T3-L1 whole cell lysate (40ug)
Lane 2: AML-12 whole cell lysate (40ug)
Lane 3: H1792 whole cell lysate (40ug)
Lane 4: L02 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of TG1019 (R251) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 46 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Oxoeicosanoid receptor 1; 5-oxo-ETE G-protein coupled receptor; G-protein coupled receptor 170; G-protein coupled receptor R527; G-protein coupled receptor TG1019; OXER1; GPR170; TG1019

Immunogen: Synthetic peptide, corresponding to amino acids 220-270 of Human TG1019.

Conjugate: Unconjugated

Modification: Unmodified