Product Description

TGase2 (D11) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1367-50, BS1367-100

Sizes: 50µl, 100µl

Swiss-Prot: P21980

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Terminally differentiating mammalian epidermal cells acquire an insoluble, 10 to 20 nm thick protein deposit on the intracellular surface of the plasma membrane known as the cross-linked cell envelope (CE). The CE is a component of the epidermis that is generated through formation of disulfide bonds and g-glutamyl-lysine isodipeptide bonds, which are formed by the action of transglutaminases (TGases). TGases are intercellularly localizing, Ca2+-dependent enzymes that catalyze the formation of isopeptide bonds by transferring an amine on to glutaminyl residues, thereby cross-linking glutamine residues and lysine residues in substrate proteins. TGases influence numerous biological processes, including blood coagulation, epidermal differentiation, seminal fluid coagulation, fertilization, cell differentiation and apoptosis. Human keratinocyte transglutaminase (TGase1) is a membrane associated, 817 amino acid protein. Human tissue transglutaminase (TGase2) is an endothelial cell specific, 687 amino acid protein.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Transglutaminase 2 (D11) polyclonal antibody detects endogenous levels of Transglutaminase 2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TGase2 (D11) pAb at 1:500 dilution
Lane 1: C6 whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: SGC7901 whole cell lysate (40ug)
Lane 4: HEK293T whole cell lysate (40ug)
Lane 5: HepG2 whole cell lysate (40ug)
Lane 6: HCT116 whole cell lysate (40ug)
Lane 7: L02 whole cell lysate (20ug), Immunohistochemistry (IHC) analyzes of TGase2 (D11) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 77 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: TGM2; Protein-glutamine gamma-glutamyltransferase 2; Tissue transglutaminase; Transglutaminase C; Tg (C); TGC; TGase C; Transglutaminase H; TGase H; Transglutaminase-2; TGase-2

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human TGase2.

Conjugate: Unconjugated

Modification: Unmodified