Product Description

TGS1 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS70955-50, BS70955-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96RS0

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7G caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7G cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs. Plays a role in transcriptional regulation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using TGS1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Bioworld Molecular Weight: 97kDa

Specificity: Polyclonal Antibodies

Alternative Name: TGS1; NCOA6IP; PIMT; PIPMT

Immunogen: Recombinant fusion protein of human TGS1(NP_079107.6).

Conjugate: Unconjugated

Modification: Unmodified