TH Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91341-50, BS91341-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P07101(Human) P24529(Mouse) P04177(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC FC
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-dopa, which is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin. TH is thought to play a role in the pathogenesis of Parkinson's disease, which is associated with reduced dopamine levels. Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. TH is also known to be upregulated by the glia maturation factor (GMF), a Cdc 10/SWI6 motif-containing protein called V-1, and a variety of additional compounds.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: TH polyclonal antibody detects endogenous levels of TH protein.
Bioworld Molecular Weight: 58 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Tyrosine Hydroxylase on PC-12 cells lysates using anti-Tyrosine Hydroxylase antibody at 1/1,000 dilution., ICC staining Tyrosine Hydroxylase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: TH, Dystonia 14, DYT14, DYT5b, EC 1.14.16.2, OTTHUMP00000011225, OTTHUMP00000011226, ple, Protein Pale, TH, The, TY3H, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, Tyrosine 3-hydroxylase, Tyrosine 3-monooxygenase, Tyrosine hydroxylase,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified