Thioredoxin 1 (Trx1/TXN) Polyclonal Antibody-BS70837

Thioredoxin 1 (Trx1/TXN) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS70837-50, BS70837-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P10599

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200 | IP,1:20 - 1:50

Background: The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 12kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Thioredoxin 1) antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunohistochemistry of paraffin-embedded human colon using Thioredoxin 1) Rabbit pAb at dilution of 1:300. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunofluorescence analysis of NIH-3T3 cells using Thioredoxin 1) Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug Thioredoxin 1) antibody. Western blot was performed from the immunoprecipitate using Thioredoxin 1) antibody at a dilution of 1:500.

Alternative Name: TXN; TRDX; TRX; TRX1

Immunogen: A synthetic peptide of human (Trx1/Thioredoxin 1 (Trx1/TXN))(NP_003320.2).

Conjugate: Unconjugated

Modification: Unmodified