Product Description

THRB (L37) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1365-50, BS1365-100

Sizes: 50µl, 100µl

Swiss-Prot: P10828

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Thyroid hormone nuclear receptors (TRs) are ligand-dependent transcription factors which regulate growth, differentiation and development and represent members of the steroid/retinoic acid superfamily. The two genes encoding TRs identified to date, TRα and TRβ, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) with half-site binding motifs in the orientation of palindromes, direct repeats or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3'-triiodo-L-thyronine (T3). Transcriptional regulation by TRs is also modulated by heterodimerization with TR nuclear accessory proteins, the most extensively characterized of which are the retinoid X receptors (RXRα, RXRβ and RXRγ). To a certain extent, this activity is regulated by differential phosphorylation of TRs. Thus, not only are the biological activities of TRs regulated by heterodimerization with RXRs, but in addition, the gene regulatory activities of TRs are linked to other hormonal pathways.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Thyroid Hormone Receptor β (L37) polyclonal antibody detects endogenous levels of Thyroid Hormone Receptor β protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of THRB (L37) polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: HCT116 whole cell lysate (40ug)
Lane 3: BV2 whole cell lysate (40ug)
Lane 4: PC12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of THRB (L37) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 52 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Thyroid hormone receptor beta; Nuclear receptor subfamily 1 group A member 2; c-erbA-2; c-erbA-beta; THRB; ERBA2; NR1A2; THR1

Immunogen: Synthetic peptide, corresponding to amino acids 1-50 of Human THRB.

Conjugate: Unconjugated

Modification: Unmodified