thymidine phosphorylase Polyclonal Antibody-BS65869

thymidine phosphorylase Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65869-50, BS65869-100

Swiss-Prot: P19971

Host: Rabbit

thymidine phosphorylase Polyclonal Antibody-BS65869

Storage and Stability: Store at 4°C short term. Aliquot and store at -42°C long term. Avoid freeze-thaw cycles.

Reactivity: Human,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: In the presence of inorganic orthophosphate, the platelet-derived endothelial growth factor (PD-ECGF) thymidine phosphorylase (TP) (gliostatin) catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites.

Purification and Purity: affinity purified by Protein A

Specificity: thymidine phosphorylase Polyclonal Antibody detects endogenous levels of thymidine phosphorylase protein.

Bioworld Molecular Weight: ~50kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TYPH) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TYPH) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

Alternative Name: ECGF 1; PDEGF; ECGF1; Endothelial cell growth factor 1; Endothelial cell growth factor 1 platelet derived; Gliostatin; hPD ECGF; MNGIE; PD ECGF; PDECGF; PDEGF; Platelet derived endothelial growth factor; TdRPase; TP; TYMP; Thymidine Phosphorylase; TYPH_HUMAN; TdRPase.

Immunogen: KLH conjugated synthetic peptide derived from human Thymidine Phosphorylase:121-220/482

Modification: Phosphorylation

Conjugate: Unconjugated