Thyroid Peroxidase Monoclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: MB66022-50, MB66022-100 Product: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Swiss-Prot: P07202 Host: Mouse Reactivity: Human Applications: IHC All Applications: IHC (1/100 - 1/300) Background: The synthesis of thyroid hormones is an oxidative process that produces reactive oxygen species and requires Thyroperoxidase (TPO), a hemoprotein that is one of the major autoantigens involved in autoimmune thyroid diseases. Thyroperoxidase is a 933 amino acid, type I transmembrane glycoprotein that plays a key role in thyroid hormone synthesis and autoimmunity. TPO catalyzes the iodination of proteins, therefore causing iodide retention within thyroid cells. The ecto-domain of Thyroperoxidase includes a large N-terminal myeloperoxidase-like domain, followed by a complement control protein domain and an epidermal growth factor-like domain. Thyroperoxidase also mediates the organification and intracellular retention of radioiodide, which may lead to rapid tumor cell death. Mutations of the Thyroperoxidase gene commonly lead to goitrous congenital hypothyroidism, the most severe and frequent abnormality in thyroid iodide organification defect (IOD), in which iodide in the thyroid gland cannot be oxidized and/or bound to the protein. Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Recognizes endogenous levels of Thyroid Peroxidase protein. Bioworld Molecular Weight: ~ 102 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Immunohistochemical analysis of Thyroid Peroxidase staining in human follicular thyroid carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of Thyroid Peroxidase staining in human papillary thyroid carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Alternative Name: Thyroid peroxidase; TPO Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Thyroid Peroxidase. The exact sequence is proprietary. Conjugate: Unconjugated Modification: Unmodified