Thyroid Peroxidase Monoclonal Antibody Sizes: 50μl, 100μl Catalogue Numbers: MB66022-50, MB66022-100 Lead times: 1-2 weeks, if manufacturer has product in stock Manufacturer/Ship Location: China Background: The synthesis of thyroid hormones is an oxidative process that produces reactive oxygen species and requires Thyroperoxidase (TPO), a hemoprotein that is one of the major autoantigens involved in autoimmune thyroid diseases. Thyroperoxidase is a 933 amino acid, type I transmembrane glycoprotein that plays a key role in thyroid hormone synthesis and autoimmunity. TPO catalyzes the iodination of proteins, therefore causing iodide retention within thyroid cells. The ecto-domain of Thyroperoxidase includes a large N-terminal myeloperoxidase-like domain, followed by a complement control protein domain and an epidermal growth factor-like domain. Thyroperoxidase also mediates the organification and intracellμlar retention of radioiodide, which may lead to rapid tumor cell death. Mutations of the Thyroperoxidase gene commonly lead to goitrous congenital hypothyroidism, the most severe and frequent abnormality in thyroid iodide organification defect (IOD), in which iodide in the thyroid gland cannot be oxidized and/or bound to the protein. Category: Primary Antibody Reactivity: Human Host: Mouse Applications: IHC Alternate Names: Thyroid peroxidase, TPO Clonality: Monoclonal Conjugate: Unconjugated Modification: Unmodification Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Thyroid Peroxidase. The exact sequence is proprietary. Dilution: IHC (1/100 - 1/300) Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Specificity: Recognizes endogenous levels of Thyroid Peroxidase protein. Molecular Weight: ~ 102 kDa Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. SwissProt: P07202 Product: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Research Use Only