Product Description

TIA1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91347-50, BS91347-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P31483(Human) P52912(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
IP:1:10-1:50

Background: FAS, also referred to as CD95 or APO-1, is a type I transmembrane protein that plays a central role mediating viral immunity. TIA-1 and TIAR are two closely related proteins that possess three RRMs (RNA recognition motifs), designated RRM 1, 2 and 3. Although both TIA-1 and TIAR are thought to function as mediators of apoptotic cell death, their specific roles in such pathways are unknown. Unlike TIA-1, which is found in the granules of cytotoxic lymphocytes, TIAR expression is limited to the nucleus and found in a much broader range of cells including, but not limited to, cells of hematopoietic origin. TIAR is translocated to the cytoplasm shortly after FAS ligation and this event immediately proceeds the onset of DNA fragmentation. A novel serine/threonine kinase that is activated as a result of FAS ligation, designated FAST (FAS-activated serine/threonine), shows kinase specificity towards both TIA-1 and TIAR. In unstimulated Jurkat cells, FAST resides in the cytoplasm as a highly phosphorylated protein and is quickly dephosphorylated and activated in response to stimulated FAS.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: TIA1 polyclonal antibody detects endogenous levels of TIA1 protein.

Bioworld Molecular Weight: 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TIA1 on Jurkat cells lysates using anti-TIA1 antibody at 1/500 dilution., ICC staining TIA1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: TIA-1, Cytotoxic granule associated RNA binding protein 1, Cytotoxic granule associated RNA binding protein, mTIA-1, Nucleolysin TIA 1 isoform p40, Nucleolysin TIA-1 isoform p40, Nucleolysin TIA1 isoform p40, p40 TIA 1, p40-TIA-1 (containing p15-TIA-1), p40-TIA-1, RNA binding protein TIA 1, RNA binding protein TIA1, RNA-binding protein TIA-1, T-cell-restricted intracellular antigen-1, TIA 1, TIA 1 cytotoxic granule associated RNA binding protein, Tia, TIA-1, TIA1, TIA1 cytotoxic granule associated RNA binding protein, TIA1 cytotoxic granule associated RNA binding protein like 1, TIA1 protein, TIA1, TIAL1, TIAR, WDM,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified