Product Description

TIF-IA(Ab-649)Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65250-50, BS65250-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: Q9NYV6

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-P, IF (paraffin section), ELISA

All Applications: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Background: function:Required for efficient transcription initiation by RNA polymerase I.,similarity:Belongs to the RRN3 family.,

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phospho-Rrn3 (S649) Polyclonal Antibody detects endogenous levels of Rrn3 protein only when phosphorylated at S649.

Bioworld Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TIF-IA (Phospho-Ser649) Antibody, Immunohistochemistry analysis of paraffin-embedded human brain, using TIF-IA (Phospho-Ser649) Antibody. The picture on the right is blocked with the phospho peptide., Western blot analysis of lysates from Jurkat cells treated with starved 24h, using TIF-IA (Phospho-Ser649) Antibody. The Lane on the right is blocked with the phospho peptide.

Alternative Name: RRN3; TIFIA; RNA polymerase I-specific transcription initiation factor RRN3; Transcription initiation factor IA; TIF-IA

Immunogen: The antiserum was produced against synthesized peptide derived from human TIF-IA around the phosphorylation site of Ser649. AA range:602-651

Conjugate: Unconjugated

Modification: Unmodified