Product Description

TIF1 gamma Monoclonal Antibody

Catalogue Numbers: MB66498-50, MB66498-100

Sizes: 50µl, 100µl

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q9UPN9

Host: Mouse

Reactivity: Human

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: The transcriptional intermediary factor 1 (TIF1) family represents a group of proteins with multiple histone-binding domains. In humans, this family comprises four proteins, TIF1α/TRIM24, TIF1β/TRIM28/KAP1, TIF1γ/TRIM33/Ectodermin, and TIF1δ/TRIM66, which are characterized by an amino-terminal tripartite motif (TRIM) domain consisting of a RING domain, two B boxes, a coiled-coil domain, and a carboxy-terminal PHD finger and bromodomain . Despite their similar overall structure, these proteins have diverse roles in transcriptional regulation. TIF1α functions as a ligand-dependent nuclear receptor coregulator and more recently has been implicated in regulating p53 stability . TIF1β is an intrinsic component of the N-CoR1 corepressor complex and the NuRD nucleosome-remodeling complex and functions as a corepressor for Kruppel-associated box (KRAB) zinc-finger transcription factors. Furthermore, TIF1β promotes heterochromatin-mediated gene silencing formation by serving as a cofactor for heterochromatin protein HP1 . TIF1δ expression is restricted to the testis and has been shown to interact with HP1γ .

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of TIF1 gamma protein.

Extra Notes: Western blot analysis of TIF1 gamma expression in MCF7 (A), Hela (B) whole cell lysates.

Bioworld Molecular Weight: ~ 150 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: KIAA1113; RFG7; TIF1G; E3 ubiquitin-protein ligase TRIM33; Ectodermin homolog; RET-fused gene 7 protein; Protein Rfg7; Transcription intermediary factor 1-gamma; TIF1-gamma; Tripartite motif-containing protein 33

Immunogen: Purified recombinant human TIF1 gamma / TRIM33 protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified