Tight Junction Protein ZO 3 Monoclonal Antibody-MB11799

Tight Junction Protein ZO 3 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11799-50, MB11799-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: O95049

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB: 1/500-1/1000

Background: TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:16129888). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions (PubMed:16129888). Promotes cell-cycle progression through the sequestration of cyclin D1 (CCND1) at tight junctions during mitosis which prevents CCND1 degradation during M-phase and enables S-phase transition (PubMed:21411630). With TJP1 and TJP2, participates to the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus . Contrary to TJP2, TJP3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment .

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Zonula occludens protein 3 in MCF-7, Caco2Lysates using Tight Junction Protein ZO 3 antibody.

Bioworld Molecular Weight: Calculated MW: 101 kDa; Observed MW: 140 kDa

Specificity: IgG

Alternative Name: ZO3; ZO-3

Immunogen: A synthetic peptide of human Zonula occludens protein 3

Conjugate: Unconjugated

Modification: Unmodified