Product Description

Timm29 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS78108-50, BS78108-100

Swiss-Prot: Q8BGX2

Host: Rabbit

Timm29 Polyclonal Antibody-BS78108

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:200

Background: Component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as the external driving force. Required for the stability of the TIM22 complex and functions in the assembly of the TIMM22 protein into the TIM22 complex. May facilitate cooperation between TIM22 and TOM complexes by interacting with TOMM40.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 29KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Timm29 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s., Immunoprecipitation analysis of 300ug extracts of A-549 cells using 3ug Timm29 antibody. Western blot was performed from the immunoprecipitate using Timm29 antibody at a dilution of 1:1000.

Alternative Name: TIM29; 1810026J23Rik

Immunogen: Recombinant fusion protein of mouse Timm29(NP_848734.1).

Modification: Unmodified

Conjugate: Unconjugated