Tissue Factor Polyclonal Antibody-BS91349

Tissue Factor Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91349-50, BS91349-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P13726(Human) P20352(Mouse) P42533(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:100-1:500

Background: Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which are involved in a blood coagulation cascade leading to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Coagulation Factor V (Factor V, FV, proaccelerin, labile factor) is a 2196 amino acid, single chain glycoprotein that is cleaved by Thrombin to yield an active, Ca2+-dependent dimer that is essential to the blood coagulation cascade. Together with catalytic Factor Xa and Ca2+ on the surface of platelets or endothelial cells, Factor Va coordinates into a Prothrombinase complex, which mediates proteolysis of Prothrombin into active Thrombin. Tissue factor (TF), also designated coagulation Factor III is a cell surface glycoprotein that enables cells to initiate blood coagulation cascades. It functions as a high-affinity receptor for coagulation Factor VII.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Tissue Factor polyclonal antibody detects endogenous levels of Tissue Factor protein.

Bioworld Molecular Weight: 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Tissue Factor on different lysates using anti-Tissue Factor antibody at 1/1,000 dilution.
Positive control:
Lane 1: U937
Lane 2: SH-SY-5Y
Lane 3: Mouse brain, ICC staining Tissue Factor in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: TF, F3, CD142, CD142 antigen, Coagulation factor III (thromboplastin tissue factor), Coagulation factor III, F3, FLJ17960, TF, TF, TFA, Thromboplastin, Tissue factor,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified