TK Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91350-50, BS91350-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P04183(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Thymidine Kinase (TK1) is a highly conserved phosphotransferase that is present in most living cells. Thymidine Kinase catalyzes the phosphorylation reaction: deoxythymidine + ATP = deoxythymidine 5'-phosphate + ADP; it is thus involved in the reaction chain to introduce deoxythymidine into the DNA. Thymidine kinase is required for the action of many antiviral drugs, such as azidothymidine (AZT), and is is also used to select hybridoma cell lines in the production of monoclonal antibodies. Thymidine Kinase has many clinical applications as it is only present in anticipation of cell division. Because of this, Thymidine Kinase can be used as a proliferation marker in the diagnosis, treatment, and follow-up of malignant diseases, especially hematological malignancies. Thymidine Kinase may be observed as a monomer, dimer, trimer or tetramer.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: TK polyclonal antibody detects endogenous levels of TK protein.
Bioworld Molecular Weight: 25 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Thymidine Kinase 1 on different lysates using anti-Thymidine Kinase 1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Hela, ICC staining Thymidine Kinase 1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: TK1, cytosolic, KITH, Thymidine kinase 1, Thymidine kinase 1 soluble, Thymidine kinase 1 soluble isoform, Thymidine kinase, Thymidine kinase cytosolic, TK 1, TK 2, TK1, Tk1a, Tk1b, TK2,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified