TLE1/2/3/4 rabbit pAb - ES8464

TLE1/2/3/4 rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES8464-50, ES8464-100

Citations, Manuals and MSDS Available upon request.

Background: function:Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG., PTM:Phosphorylated, probably by CDC2. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1., similarity:Belongs to the WD repeat Groucho/TLE family., similarity:Contains 6 WD repeats., subcellular location:Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components., subunit:Homooligomer and heterooligomer with other family members. Binds LEF1, RUNX1, RUNX3, FOXA2, KDM6A, UTY, histone H3, HESX1, ESRRG and the NF-kappa-B subunit RELA. Interacts with HES1 (via WRPW motif)., tissue specificity:In all tissues examined, mostly in brain, liver and muscle.,

Alternate Name: similar to transducin-like enhancer of split 1/2/3/4

Source: Rabbit

Applications: WB; ELISA; IHC

Dilution: WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000

Reactivity: Human; Mouse; Rat

Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human TLE1/TLE2/TLE3/TLE4. AA range:721-770

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 90kD

Human Gene ID: 7088

Human SWISS Prot NO: Q04724/Q04725/Q04726/Q04727

Subcellular Location: Nucleus. Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.

Research Use Only