TLE1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS75306-50, BS75306-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q04724
Host: Rabbit
Reactivity: Mouse
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Enhances FOXG1/BF-1- and HES1-mediated transcriptional repression (By similarity. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of Mouse liver, using TLE1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 83kDa
Alternative Name: TLE1; ESG; ESG1; GRG1
Immunogen: Recombinant fusion protein of human TLE1(NP_005068.2).
Conjugate: Unconjugated
Modification: Unmodified