Product Description

TLE4 (H190) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2035-50, BS2035-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q04727

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:10~1:100

Background: TLEs associate with chromatin in live cells and specifically with Histone H3, but not with other core histones. Expression of the TLE genes, TLE1, TLE2, TLE3 and TLE4, correlate with immature epithelial cells that are progressing toward a terminally differentiated state, suggesting a role during epithelial differentiation. TLE1, TLE2 and TLE3 have elevated expression in cervial squamous metaplasias and carcinomas, while TLE4 is most highly expressed in the brain, particularly in the caudate nucleus. TLE1 and TLE4 contain SP and WD40 domains, through which TLE1 binds AML1 to inhibit AML1-induced transactivation of the CSF1 receptor. In early stages of cell differentiation, TLE1 is up-regulated, and TLE2 and TLE4 are down-regulated.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: TLE4 (H190) polyclonal antibody detects endogenous levels of TLE4 protein.

Bioworld Molecular Weight: ~ 83 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TLE4 (H190) pAb at 1:500 dilution
Lane 1: U-87MG whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (10ug)
Lane 3: The Testis tissue lysate of Mouse (40ug)
Lane 4: The Testis tissue lysate of Rat (40ug), Immunoprecipitation of HEK293T cell lysate using TLE4 (H190) pAb (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using TLE4 (H190)#BS2035. “↑”（supernatant); “↓(deposition）

Alternative Name: Transducin-like enhancer protein 4; TLE-4; KIAA1261

Immunogen: Synthetic peptide, corresponding to amino acids 170-230 of Human TLE4.

Conjugate: Unconjugated

Modification: Unmodified