Tle6 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91351-50, BS91351-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9H808(Human) Q9WVB3(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC IHC FC
All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: The Notch signaling pathway controls various cellular interactions that are important for the specification of a variety of fates in both vertebrates and invertebrates. Key players in the Notch pathway are the TLE genes (for transducin-like enhancer of split, also designated ESG for enhancer of split groucho), which are human homologs of the Drosophila groucho gene. TLE6 (transducin-like enhancer of split 6), also known as GRG6, is a 449 amino acid cytoplasmic protein belonging to the WD repeat groucho/TLE family. As a member of the subcortical maternal complex (SCMC), TLE6 is essential for zygotes to progress beyond the first embryonic cell divisions. TLE6 contains seven WD repeats, a motif known to mediate protein-protein interactions. The WD40 repeat family of proteins is suggested to be involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation.
Purification and Purity: Peptide affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: Tle6 polyclonal antibody detects endogenous levels of Tle6 protein.
Bioworld Molecular Weight: 63 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Tle6 on different lysates using anti-Tle6 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse ovary tissue
Lane 2: HepG2, ICC staining Tle6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: TLE6,
Immunogen: Synthetic peptide within mouse Tle6 aa 150-200.
Conjugate: Unconjugated
Modification: Unmodified