TLK1 Polyclonal Antibody-BS62542

TLK1 Polyclonal Antibody

Catalogue Numbers: BS62542-50, BS62542-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UKI8

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: The Tousled-like kinases (TLK1 and TLK2, also designated PKU-β and PKU-α, respectively) are the human homologs of the Tousled gene from Arabidopsis thaliana, which encodes a Serine/Threonine kinase that is necessary for proper organ morphogenesis. Both TLKs contain a nuclear localization signal and a predicted coiled-coil region in the N-terminal domain. TLK is ubiquitously expressed, and is prevalent in mouse testis, especially in pachytene spermatocytes and round spermatids. It displays a propensity to dimerize through an interaction between its coiled-coil structure and is able to autophosphorylate, as well as phosphorylate exogenous substrates. TLK1 and TLK2 are regulated by the cell cycle, showing maximum activity during S phase. Subsequently, they are thought to regulate the development of multicellular organisms, including playing a key role in spermatogenesis, through a series of phosphorylations.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: TLK1 polyclonal antibody detects endogenous levels of TLK1 protein.

Extra Notes: Western blot (WB) analysis of TLK1 polyclonal antibody at 1:500 dilution
LaneA:HepG2 whole cell lysate
LaneB:AML-12 whole cell lysate

Bioworld Molecular Weight: ~ 87 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Serine/threonine-protein kinase tousled-like 1; PKU-beta; Tousled-like kinase 1; TLK-1; KIAA0137; PKU-β; PKU β; PKUβ

Immunogen: Synthetic peptide, corresponding to Human TLK1.

Conjugate: Unconjugated

Modification: Unmodified