Product Description

TLR4 (S441) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3489-50, BS3489-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00206

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF, IP,

All Applications: WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200

Background: Toll like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti fungal response, while the related 18 wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram negative bacteria.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: TLR4 (S441) polyclonal antibody detects endogenous levels of TLR4 protein.

Bioworld Molecular Weight: ~ 120 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TLR4 (S441) pAb at 1:500 dilution
Lane 1: HCT116 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: Myla2059 whole cell lysate (40ug)
Lane 4: Jurkat whole cell lysate (40ug)
Lane 5: MCF-7 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of TLR4 (S441) pAb in paraffin-embedded human brain tissue., Immunoprecipitation of MCF-7 cell lysates using TLR4 (S441) pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using TLR4 (S441) pAb #BS3489.

Alternative Name: Toll-like receptor 4; hToll; CD284; TLR-4

Immunogen: Synthetic peptide, corresponding to amino acids 421-470 of Human TLR4.

Conjugate: Unconjugated

Modification: Unmodified