Product Description

TMPase Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66098-50, MB66098-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Prostatic Acid Phosphatase (ACPP or PAP) is a member of the histidine acid phosphatase family. It is a non-specific phosphatase that is capable of dephosphorylating tyrosine residues as well as phospholipids under mildly acidic conditions. ACPP has ecto-5'-nucleotidase activity in pain-sensing neurons where it converts AMP to adenosine, suppressing the pain response. ACPP occurs as two isoforms that are both heavily glycosylated. The secreted phosphatase (sPAP) is found predominantly in the prostate and seminal plasma, while the cellμlar isoform (cPAP) is broadly expressed at very low levels and is associated with the plasma and lysosomal membranes. Cellμlar PAP has been shown to dephosphorylate ErbB2 at various tyrosine residues effectively terminating signaling. Furthermore, the physical interaction between cPAP and ErbB2 appears to regμlate androgen sensitivity in prostate cancer cells. Loss of cPAP in androgen-sensitive prostate cancer cells resμlts in the development of a castration-resistant phenotype suggesting that ACPP plays a significant role in prostate cancer cell growth. ACPP is expressed in metastatic cells arising from prostate cancer - especially in prostate-derived bone metastasis - suggesting that it may be a relevant diagnostic indicator of prostate cancer re-emergence in bone.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: IHC

Alternate Names: Prostatic acid phosphatase, PAP, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, Thiamine monophosphatase, TMPase

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human TMPase. The exact sequence is proprietary.

Dilution: IHC (1/100 - 1/300)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of TMPase protein.

Molecular Weight: ~ 44 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P15309

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Research Use Only