Product Description

TNAP Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9818M-50, BS9818M-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P05186

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IHC, IP, IF

All Applications: WB: 1:500-1:2000
IHC/ICC: 1:50-1:100
IP: 1:20-1:50
IF: 1:50-1:100

Background: Alkaline phosphatases (AP) are glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallated glycoproteins that catalyze the hydrolysis of phospho-monoesters into an inorganic phosphate and an alcohol. There are at least four distinct but related alkaline phosphatases: intestinal (IAP), placental (PLAP), placental-like (ALP-1 or GCAP) and tissue non-specific (TNAP). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. TNAP is widely expressed in liver, kidney, bone, stomach and colon, and is therefore referred to as the tissue non-specific form of AP. TNAP, in conjuntion with plasma cell membrane glycoprotein-1, function in bone mineralization; however, mice that lack a functional form of TNAP show normal skeletal development.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: TNAP monoclonal antibody detects endogenous levels of TNAP protein.

Bioworld Molecular Weight: ~ 72 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Primary: Anti-Alkaline Phosphatase at 1/1000 dilution, Tissue: Human liver
Section type: Formalin-fixed and Paraffin
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100

Alternative Name: Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme; ALPL

Immunogen: KLH conjugated synthetic peptide derived from human Alkaline Phosphatase: 18-50

Conjugate: Unconjugated

Modification: Unmodified