Product Description

TNF-α (Q178) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1857-50, BS1857-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimμlated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a mμltimer of two, three, or five noncovalently linked units, but shows a single 17 a band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 a protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellμlar actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cμltured normal human cells, TNF alpha appears to be directly toxic to vascμlar endothelial cells.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2, TNFα, TNF α

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TNF alpha.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TNFα (Q178) polyclonal antibody detects endogenous levels of TNFα protein.

Molecular Weight: ~ 17, 26 kDa

Pathway: Necrotic Cell Death,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P01375

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only