Product Description

TNF-β (H66) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3491-50, BS3491-100

Sizes: 50µl, 100µl

Swiss-Prot: P01374

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC

Background: Tumor necrosis factor b (TNFb), also known as lymphotoxin, is a pleiotropic cytokine. TNFa, also known as cachectin, is a smaller cytokine that binds to the same receptors, producing a vast array of effects similar to those of TNFb. TNFb and TNFa share 30% amino acid homology and have similar biological activities. TNFb is produced by activated lymphocytes, including CD4(+) T helper cell type 1 lymphocytes, CD8(+) lymphocytes and certain B lymphoblastoid cell lines. TNFa is produced by several different cell types, including lymphocytes, neutrophils and macrophages. TNFa and TNFb can modulate many immune and inflammatory functions, while having the ability to inhibit tumor growth.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TNF-β (H66) polyclonal antibody detects endogenous levels of TNF-β protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TNF-β (H66) pAb at 1:500 dilution
Lane 1: The Testis tissue lysate of Rat (20ug)
Lane 2: A549 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (20ug)
Lane 4: PC3 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of TNF-β (H66) pAb in paraffin-embedded human kidney tissue at 1:100.

Bioworld Molecular Weight: ~ 22 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Lymphotoxin-alpha; LT-alpha; TNF-beta; Lymphotoxin α; LTα; TNFβ; Tumor necrosis factor ligand superfamily member 1; LTA; TNFB; TNFSF1

Immunogen: Synthetic peptide, corresponding to amino acids 31-80 of Human TNF-β.

Conjugate: Unconjugated

Modification: Unmodified