Product Description

TNF alpha Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16028-50, BZ16028-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P01375

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Background: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 26 kDa; Observed MW: 26 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TNF alpha in mouse liver lysates using TNF alpha antibody., Immunohistochemistry analysis of paraffin-embedded Human heart using TNF alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunohistochemistry analysis of paraffin-embedded Human heart using TNF alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunohistochemistry analysis of paraffin-embedded Human colon using TNF alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human TNF. AA range:151-200

Conjugate: Unconjugated

Modification: Unmodified