Product Description

TNF alpha Rat Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB65864-50, MB65864-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein. Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer. Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages. Cellμlar response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and resμlts in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death. TNF-α plays a key regμlatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tubercμlosis. Mouse and rat TNF-α are predicted to be glycosylated, while human TNF-α is not.

Category: Primary Antibody

Reactivity: Mouse

Host: Rat

Applications: IF, FC

Alternate Names: TNFA, TNFSF2, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Dilution: IF (1/50 - 1/200)

Purification: The monoclonal antibody was affinity-purified from rat antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes mouse TNF alpha

Molecular Weight: ~ 25 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P06804

Product: Rat IgG1 kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Research Use Only