TNFR2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91362-50, BS91362-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P20333(Human) P25119(Mouse) Q80WY6(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, FC, IP,
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50
Background: Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors. These receptors, designated TNF-R1 and TNF-R2, are expressed on most cell types. The majority of TNF functions are primarily mediated through TNF-R1, while signaling through TNF-R2 occurs less extensively and is confined to cells of the immune system. Both of these proteins belong to the growing TNF and nerve growth factor (NGF) receptor superfamily, which includes FAS, CD30, CD27 and CD40. The members of this superfamily are type I membrane proteins that share sequence homology confined to the extracellular region. TNF-R1 shares a motif termed the "death domain" with FAS and three structurally unrelated signaling proteins, TRADD, FADD and RIP (1,3-8). This death domain is required for transduction of the apoptotic signal.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: TNFR2 polyclonal antibody detects endogenous levels of TNFR2 protein.
Bioworld Molecular Weight: 73 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of TNF Receptor II on different cells lysates using anti-TNF Receptor II antibody at 1/500 dilution.
Positive control:
Line 1: MCF-7
Line 2: Jurkat, ICC staining TNF Receptor II in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: TNF-R2, TNF-RII, TNFR-II, TNFRSF1B, CD120b, p75, p75 TNF receptor, p75TNFR, p80 TNF alpha receptor, p80 TNF-alpha receptor, Soluble TNFR1B variant 1, TBP-2, TBPII, TNF R II, TNF R2, TNF R75, TNF-R2, TNF-RII, TNFBR, TNFR-II, TNFR1B, TNFR2, TNFR80, TNFRII, Tnfrsf1b, TNR1B, Tumor necrosis factor beta receptor, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, Tumor necrosis factor-binding protein 2,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified