Product Description

tNOX Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67327-50, BS67327-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q16206

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB (1/500 - 1/2000), IF/ICC (1/50 - 1/100)

Background: May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of tNOX protein.

Bioworld Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of tNOX expression in SW480 (A), Jurkat (B), mouse kidney (C), mouse ovary (D), rat liver (E) whole cell lysates., Immunofluorescent analysis of tNOX staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: COVA1; Ecto-NOX disulfide-thiol exchanger 2; APK1 antigen; Cytosolic ovarian carcinoma antigen 1; Tumor-associated hydroquinone oxidase; tNOX

Immunogen: Recombinant full length protein of human tNOX

Conjugate: Unconjugated

Modification: Unmodified