Product Description

TOM20 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80485-50, BS80485-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15388

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:20 - 1:50 | IP,1:50 - 1:200

Background: Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 16KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug TOM20 antibody. Western blot was performed from the immunoprecipitate using TOM20 antibody at a dilution of 1:1000., Immunohistochemistry of paraffin-embedded Mouse kidney using TOM20 Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded Rat kidney using TOM20 Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded Human colon using TOM20 Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Alternative Name: TOMM20; MAS20; MOM19; TOM20

Immunogen: Recombinant fusion protein of human SETDB1(NP_001230420.1).

Conjugate: Unconjugated

Modification: Unmodified