Product Description

TOMM20 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS66151-50, BS66151-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The mitochondrial preprotein translocases of the outer membrane (Tom) is a mμltisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP. In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP. Tom22 directly associates with Tom40, the major component of the GIP, and thereby forms a stable interaction between the two core complexes to facilitate the fluid movement of preproteins into the mitochondria. The insertion of Tom40 into the Tom machinery requires the initial binding of Tom40 to Tom20 and leads to the efficient incorporation of Tom40 precursors into preexisting Tom complexes.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA, IHC-P

Alternate Names: TOM20, KIAA0016, MAS20, MGC117367, Mitochondrial 20 kDa outer membrane protein, Mitochondrial import receptor subunit TOM20 homolog, MOM19, Outer mitochondrial membrane receptor Tom20, TOM 20, TOM20_HUMAN, Translocase of outer mitochondrial membrane 20 homolog (yeast), Translocase of outer mitochondrial membrane 20 homolog type II

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH conjugated synthetic peptide derived from human TOM20: 1-100/145

Dilution: WB: 1:500-1:2000
ELISA: 1:5000-1:10000
IHC-P: 1:100-1:500

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TOMM20 polyclonal antibody detects endogenous levels of TOMM20 protein.

Molecular Weight: ~ 16 kDa

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

SwissProt: Q15388

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Research Use Only