TOP1 Polyclonal Antibody-BS91366

TOP1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91366-50, BS91366-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11387(Human) Q04750(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: DNA topoisomerases play essential roles in many DNA metabolic processes including DNA repair. Topoisomerases can introduce DNA damage upon exposure to drugs that stabilize the covalent protein-DNA intermediate of the topoisomerase reaction. Lesions in DNA are also able to trap topoisomerase-DNA intermediates. DNA topoisomerase I (Top1) catalyzes the relaxation of supercoiled DNA by a mechanism of transient DNA strand cleavage characterized by the formation of a phosphotyrosyl bond between the DNA end and active site tyrosine. The antitumor agent camptothecin (CPT) reversibly stabilizes the covalent enzyme-DNA intermediate by inhibiting DNA religation. When a replication fork collides with a DNA Top1 cleavage complex, the covalently bound enzyme must be removed from the DNA 3' end before recombination-dependent replication restart. The tyrosyl-DNA phosphodiesterase Tdp1 and the structure-specific endonuclease Rad1-Rad10 function as primary alternative pathways of Top1 repair in Saccharomyces cerevisiae. In the budding yeast S. cerevisiae, DNA topoisomerases I and II can functionally substitute for each other in removing positive and negative DNA supercoils.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: TOP1 polyclonal antibody detects endogenous levels of TOP1 protein.

Bioworld Molecular Weight: 91 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TOP1 on different lysates using anti-TOP1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Jurkat
Lane 3: MCF-7, ICC staining TOP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: TOP1, DNA topoisomerase 1, DNA topoisomerase I, NUP98 fusion gene, TOP 1, TOP I, TOP1, TOP1, TOPI, Topoisomerase (DNA) I, Topoisomerase 1, Topoisomerase1, TopoisomeraseI, Type I DNA topoisomerase,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified