TOP2A Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91367-50, BS91367-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P11388
Host: Rabbit
Reactivity: Human, Mouse, rat
Applications: WB, ICC IHC FC
All Applications: WB:1:500
ICC:1:200
IHC:1:200
FC:1:100-1:200
Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. It is essential during mitosis and meiosis for proper segregation of daughter chromosomes.
Purification and Purity: Peptide affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: TOP2A polyclonal antibody detects endogenous levels of TOP2A protein.
Bioworld Molecular Weight: 174 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of TOP2A on different cell lysates using anti-TOP2A antibody at 1/500 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: NIH/3T3
Lane 3: A431
Lane 4: L929
Lane 5: A549
Lane 6: Human liver
Lane 7: HUVEC, ICC staining TOP2A in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: TOP2A, alpha isozyme, ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, DNA topoisomerase II 170 kD, DNA topoisomerase II alpha isozyme, DNA topoisomerase II, DNA Topoisomerase2, TOP 2A, TOP2, TOP2A, TOP2A, Topoisomerase DNA II alpha 170kDa, TP2A,
Immunogen: peptide
Conjugate: Unconjugated
Modification: Unmodified