Product Description

TOP2A Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91368-50, BS91368-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11388(Human) Q01320(Mouse) P41516(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB:1:1,000-1:2,000
IHC:1:50-1:200

Background: DNA topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing both positive and negative supercoils, whereas prokaryotic topoisomerases relax only negative supercoils. DNA topoisomerases play a role in DNA replication, recombination, and transcription and have been identified as targets of numerous anticancer drugs. Topo I, a ubiquitously expressed, soluble enzyme, acts by introducing a transient break in one strand of DNA, while Topo II acts by making a transient double-strand break. Topo II is encoded by two different genes to generate two distinct isoforms that are designated Topo IIα and Topo IIβ. Topo IIβ and Topo IIα, are largely homologous at their N-terminal three quarters, however, the C-terminal segments are considerably divergent, suggesting that these regions may mediate different cellular functions and account for the observed differential tissue expression patterns of the two isoforms.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: TOP2A polyclonal antibody detects endogenous levels of TOP2A protein.

Bioworld Molecular Weight: 174 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Topoisomerase Ⅱ alpha on mouse testis lysates using anti-Topoisomerase Ⅱ alpha antibody at 1/1,000 dilution., Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Topoisomerase Ⅱ alpha antibody. Counter stained with hematoxylin.

Alternative Name: TOP2A, TOP2, alpha isozyme, ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, DNA topoisomerase II 170 kD, DNA topoisomerase II alpha isozyme, DNA topoisomerase II, DNA Topoisomerase2, TOP 2A, TOP2, TOP2A, TOP2A, Topoisomerase DNA II alpha 170kDa, TP2A,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified