Product Description

Topoisomerase 2 alpha Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66169-50, MB66169-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: DNA topoisomerases I and II are nuclear enzymes, type II consists of two highly homologous isoforms: topoisomerase IIα and IIβ. These enzymes regμlate the topology of DNA, maintain genomic integrity, and are essential for processes such as DNA replication, recombination, transcription, and chromosome segregation by allowing DNA strands to pass through each other. Topoisomerase I nicks and rejoins one strand of the duplex DNA, while topoisomerase II transiently breaks and closes double-stranded DNA . Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase targeting drugs (e.g., etoposide) also convert topoisomerases to nucleases, with the enzyme usually trapped as an intermediate that is covalently bound to the 5+ end of the cleaved DNA strand(s). Research studies have shown that this intermediate leads to genomic instability and cell death. Thus, agents that target topoisomerases are highly sought after cancer chemotherapeutic drugs . Ca2+-regμlated phosphorylation of topoisomerase IIα at Ser1106 modμlates the activity of this enzyme and its sensitivity to targeting drugs .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC, IF

Alternate Names: TOP2, DNA topoisomerase 2-alpha, DNA topoisomerase II alpha isozyme

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Topoisomerase 2 alpha. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/100 - 1/300), IF (1/100 - 1/500)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Topoisomerase 2 alpha protein.

Molecular Weight: ~ 174 kDa

Pathway: Cell Cycle G2 M DNA Damage Signaling Pathway,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P11388

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Research Use Only