Product Description

Topoisomerase 2 alpha (Phospho-S1213) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67431-50, BS67431-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: DNA topoisomerases I and II are nuclear enzymes, type II consists of two highly homologous isoforms: topoisomerase IIα and IIβ. These enzymes regμlate the topology of DNA, maintain genomic integrity, and are essential for processes such as DNA replication, recombination, transcription, and chromosome segregation by allowing DNA strands to pass through each other. Topoisomerase I nicks and rejoins one strand of the duplex DNA, while topoisomerase II transiently breaks and closes double-stranded DNA . Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase targeting drugs (e.g., etoposide) also convert topoisomerases to nucleases, with the enzyme usually trapped as an intermediate that is covalently bound to the 5+ end of the cleaved DNA strand(s). Research studies have shown that this intermediate leads to genomic instability and cell death. Thus, agents that target topoisomerases are highly sought after cancer chemotherapeutic drugs. Ca2+-regμlated phosphorylation of topoisomerase IIα at Ser1106 modμlates the activity of this enzyme and its sensitivity to targeting drugs.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB

Alternate Names: TOP2, DNA topoisomerase 2-alpha, DNA topoisomerase II alpha isozyme

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Topoisomerase 2 alpha with a site at pS1213. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of Topoisomerase 2 alpha with a site at pS1213 protein.

Molecular Weight: ~ 190 kDa

Pathway: Cell Cycle G2 M DNA Damage Signaling Pathway.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P11388

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only