Product Description

Topoisomerase I Polyclonal Antibody

Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16686-50, BZ16686-100

Swiss-Prot: P11387

Host: Rabbit

Topoisomerase I Polyclonal Antibody-BZ16686

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IHC-P, ICC/IF, FC

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100

Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.

Purification and Purity: Affinity Purified

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 91 kDa; Observed MW: 91 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TOP1 in JurkatLysates using Topoisomerase I antibody., Immunohistochemistry analysis of paraffin-embedded Human colon using TOP1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: TOP1; DNA topoisomerase 1; DNA topoisomerase I

Immunogen: A synthesized peptide derived from human TOP1

Modification: Unmodified

Conjugate: Unconjugated