Product Description

TPD52L3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76365-50, BS76365-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96J77

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:200 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the tumor protein D52-like family of proteins. These proteins are characterized by an N-terminal coiled-coil motif that is used to form homo- and heteromeric complexes with other tumor protein D52-like proteins. The encoded protein may play a role in spermatogenesis. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 14kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using TPD52L3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30S., Immunofluorescence analysis of rat testis using TPD52L3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of mouse testis using TPD52L3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: TPD52L3; NYDSP25; hD55

Immunogen: Recombinant fusion protein of human TPD52L3(NP_001001875.1).

Conjugate: Unconjugated

Modification: Unmodified