Product Description

TRα Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: GCP12-50, GCP12-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Thyroid hormone nuclear receptors (TRs) are ligand-dependent transcription factors which regμlate and control many metabolic and developmental processes. There are two genes encoding TRs identified to date, TRα and TRβ. TRs bind to thyroid hormone response elements (TREs) with half-site binding motifs in the orientation of palindromes, direct repeats or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3, 5, 3'-triiodo-L-thyronine (T3). Transcriptional regμlation by TRs is also modμlated by heterodimerization with TR nuclear accessory proteins, the most extensively characterized of which are the retinoid X receptors (RXRα, RXRβ and RXRγ). The TRα isoform TRα1 can display both a nuclear and undefined cytoplasmic location, and is the only TR that is imported into the mitochondrial matrix. TRα2 is a C-terminal variant of TRα1 that does not bind thyroid hormones (THs) and weakly binds DNA. TRα2 acts as a dominant negative antagonist of TH signalling.

Category: Primary Antibody

Reactivity: Human, Pig

Host: Rabbit

Applications: WB, IF

Alternate Names: Thyroid hormone receptor alpha, Nuclear receptor subfamily 1 group A member 1, V-erbA-related protein 7, EAR-7, c-erbA-1, c-erbA-alpha

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to Human TRα.

Dilution: WB: 1:2000~1:5000
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TRα polyclonal antibody detects endogenous levels of TRα protein.

Molecular Weight: ~ 58 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P10827

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only