TRAF2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91371-50, BS91371-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q12933(Human) P39429(Mouse) Unigene:105232(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Tumor necrosis factor (TNF)-activated cell signaling is mediated primarily through the TNF receptor 1 (TNF-R1) and, to a lesser extent, TNF-R2. Both TNF receptors are members of the expanding TNF receptor superfamily, which includes the FAS antigen and CD40. Potential insight into an understanding of TNF receptor-mediated signaling was provided by the identification of two related proteins, TRAF1 and TRAF2 (for TNF receptor-associated factors 1 and 2, respectively). Both function to form heterodimeric complexes and associate with the cytoplasmic domain of TNF-R2. A third member of this protein family, alternatively designated CD40 bp, CRAF1, LAP1 or TRAF3, has been identified and shown to associate with the cytoplasmic domain of CD40. The similarity between a specific region of TRAF3 with regions of TRAF1 and TRAF2 define a “TRAF-C” domain that is necessary and sufficient for CD40 binding and homodimerization.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: TRAF2 polyclonal antibody detects endogenous levels of TRAF2 protein.
Bioworld Molecular Weight: 56 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of TRAF2 on Hela cells lysates using anti-TRAF2 antibody at 1/1,000 dilution., ICC staining TRAF2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: TRAF2, E3 ubiquitin-protein ligase TRAF2, MGC:45012, OTTHUMP00000022625, OTTHUMP00000064745, TNF receptor associated factor 2, TNF receptor-associated factor 2, TNF receptor-associated protein, TRAF 2, TRAF2, TRAF2, TRAP 3, TRAP, TRAP3, Tumor necrosis factor type 2 receptor associated protein 3, Tumor necrosis factor type 2 receptor-associated protein 3,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified