Product Description

TRAF7 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7490-50, BS7490-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q6Q0C0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:1000 | IHC,1:50 - 1:100 | IF/ICC,1:50 - 1:100

Background: Tumor necrosis factor (TNF; see MIM 191160) receptor-associated factors, such as TRAF7, are signal transducers for members of the TNF receptor superfamily (see MIM 191190). TRAFs are composed of an N-terminal cysteine/histidine-rich region containing zinc RING and/or zinc finger motifs; a coiled-coil (leucine zipper) motif; and a homologous region that defines the TRAF family, the TRAF domain, which is involved in self-association and receptor binding.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 67kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using TRAF7 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunohistochemistry of paraffin-embedded human colon carcinoma using TRAF7 antibody at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunofluorescence analysis of U2OS cells using TRAF7 antibody at dilution of 1:50. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using TRAF7 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: TRAF7; RFWD1; RNF119

Immunogen: Recombinant fusion protein of human TRAF7(NP_115647.2).

Conjugate: Unconjugated

Modification: Unmodified