Product Description

Treacle (Q55) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2047-50, BS2047-100

Sizes: 50µl, 100µl

Swiss-Prot: Q13428

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Treacle is a serine/alanine-rich 1,411 amino acid nuclear phosphoprotein that interacts with upstream binding factor (UBF) and affects transcription of the ribosomal DNA gene. Treacle is also involved in early embryonic development, particularly in the craniofacial complex, and may play a role in nucleolarcytoplasmic transport. The Treacle protein contains three domains with unique N- and C-termini and a large central repeat domain. Mutations in TCOF1, the gene that encodes for Treacle, cause Treacher Collins-Franceschetti syndrome (TCS), a disorder characterized by defects in craniofacial development. Symptoms of TCS include conductive hearing loss, hypoplasia of the mandible and maxilla, downward sloping palpebral fissures and cleft palate.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Treacle (Q55) polyclonal antibody detects endogenous levels of Treacle protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Treacle (Q55) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: PC3 whole cell lysate (40ug)
Lane 3: A549 whole cell lysate (40ug)
Lane 4: PC12 whole cell lysate (40ug)
Lane 5: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Treacle (Q55) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 152 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Treacle protein; Treacher Collins syndrome protein; TCOF1

Immunogen: Synthetic peptide, corresponding to amino acids 22-73 of Human Treacle.

Conjugate: Unconjugated

Modification: Unmodified