TRF1 (L215) Polyclonal Antibody-BS1854

TRF1 (L215) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1854-50, BS1854-100

Sizes: 50µl, 100µl

Swiss-Prot: P54274

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Telomeric repeat binding factor 1 (TERF1, PIN2, TRF1, TRBF1) and 2 (TERF2, TRF2, TRBF2) are present at telomeres throughout the cell cycle where they regulate telomerase by acting in cis to limit the elongation of individual chromosome ends. Telomerase adds hexameric repeats of 5'-TTAGGG-3' to the ends of chromosomal DNA. This telomerase enzyme plays an influential role in cellular immortalization and cellular senescence. TRF1 negatively regulates telomere elongation, while TRF2 protects the chromosome ends by inhibiting end-to-end fusions. Down-regulation of TRF expression in tumor cells may contribute to cell immortalization and malignant progression. TRF1 has an acidic N-terminus while TRF2 has a basic N-terminus.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TRF1 (L215) polyclonal antibody detects endogenous levels of TRF1 protein

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TRF1 (L215) pAb at 1:1000 dilution
Lane 1: The Ovary tissue lysate of Rat (40ug)
Lane 2: The Testis tissue lysate of Mouse (10ug)
Lane 3: A2780 whole cell lysate (10ug), Immunohistochemistry (IHC) analyzes of TRF1 (L215) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 55 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Telomeric repeat-binding factor 1; NIMA-interacting protein 2; TTAGGG repeat-binding factor 1; Telomeric protein Pin2/TRF1; TERF1; PIN2; TRBF1; TRF; TRF1

Immunogen: Synthetic peptide, corresponding to amino acids 200-250 of Human TRF1.

Conjugate: Unconjugated

Modification: Unmodified