TRIB1 Polyclonal Antibody-BS70782

TRIB1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS70782-50, BS70782-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96RU8

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:1000 - 1:4000

Background: Adapter protein involved in protein degradation by interacting with COP1 ubiquitin ligase. The COP1-binding motif is masked by autoinhibitory interactions with the protein kinase domain. Serves to alter COP1 substrate specificity by directing the activity of COP1 toward CEBPA. Binds selectively the recognition sequence of CEBPA. Regulates myeloid cell differentiation by altering the expression of CEBPA in a COP1-dependent manner (By similarity. Controls macrophage, eosinophil and neutrophil differentiation via the COP1-binding domain (By similarity. Interacts with MAPK kinases and regulates activation of MAP kinases, but has no kinase activity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 38kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of extracts of various cell lines, using TRIB1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Alternative Name: TRIB1; C8FW; GIG-2; GIG2; SKIP1; TRB-1; TRB1

Immunogen: Recombinant fusion protein of human TRIB1(NP_079471.1).

Conjugate: Unconjugated

Modification: Unmodified